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URN: urn:nbn:de:kobv:517-opus-45853
URL: http://opus.kobv.de/ubp/volltexte/2010/4585/


Püschel, Gerhard ; Kirchner, C. ; Schröder, A. ; Jungermann, Kurt

Glycogenolytic and antiglycogenolytic prostaglandin E₂ actions in rat hepatocytes are mediated via different signalling pathways

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Kurzfassung auf Englisch

Prostaglandin E₂ has been reported both to stimulate glycogen-phosphorylase activity (glycogenolytic effect) and to inhibit the glucagon-stimulated glycogen-phosphorylase activity (antiglycogenolytic effect) in rat hepatocytes. It was the purpose of this study to resolve this apparent contradiction and to characterize the signalling pathways and receptor subtypes involved in the opposing prostaglandin E₂ actions.
Prostaglandin E₂ (10 μM) increased glucose output, glycogen-phosphorylase activity and inositol trisphosphate formation in hepatocyte cell culture andor suspension. In the same systems, prostaglandin E₂ decreased the glucagon-stimulated (1 nM) glycogen-phosphorylase activity and cAMP formation.
The signalling pathway leading to the glycogenolytic effect of PGE₂ was interrupted by incubation of the hepatocytes with 4P-phorbol 12-myristate 13-acetate (100 nM) for 10 min, while the antiglycogenolytic effect of prostaglandin E₂ was not attenuated.
The signalling pathway leading to the antiglycogenolytic effect of prostaglandin E₂ was interrupted by an incubation of cultured hepatocytes with pertussis toxin (100 ng/ml) for 18 h, whereas the glycogenolytic effect of prostaglandin E₂ was enhanced.
The EP₁/EP₃ prostaglandin-E₂-receptor-specific prostaglandin E₂ analogue Sulproston had a stronger glycogenolytic potency than the EP₃ prostaglandin-E₂-receptor-specific prostaglandin E₂ analogue Misoprostol. The antiglycogenolytic potency of both agonists was equal.
It is concluded that the glycogenolytic and the antiglycogenolytic effects of prostaglandin E₂ are mediated via different signalling pathways in hepatocytes possibly involving EP₁ and EP₃ prostaglandin E₂ receptors, respectively.

Institut: Institut für Ernährungswissenschaft
DDC-Sachgruppe: Biowissenschaften, Biologie
Dokumentart: c Postprint
Schriftenreihe: Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe, ISSN 1866-8372
Band Nummer: paper 112
Quelle: European Journal of Biochemistry 218 (1993), 3, p. 1083-1089, DOI 10.1111/j.1432-1033.1993.tb18468.x, ISSN 0014-2956
Sprache: Englisch
Erstellungsjahr: 1993
Publikationsdatum: 04.08.2010
Bemerkung:
first published in:
FEBS Journal - 218 (1993), 3, p. 1083-1089
ISSN: 0014-2956
doi: 10.1111/j.1432-1033.1993.tb18468.x
Lizenz: Diese Nutzungsbedingung gilt nicht, wenn in den Metadaten eine modifizierende Lizenz genannt ist. Keine Nutzungslizenz vergeben - es gilt das deutsche Urheberrecht


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