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URN: urn:nbn:de:kobv:517-opus-45007
URL: http://opus.kobv.de/ubp/volltexte/2010/4500/


Zhang, Gong ; Ignatova, Zoya

Generic algorithm to predict the speed of translational elongation : implications for protein biogenesis

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Kurzfassung auf Deutsch

Synonymous codon usage and variations in the level of isoaccepting tRNAs exert a powerful selective force on translation fidelity. We have developed an algorithm to evaluate the relative rate of translation which allows large-scale comparisons of the non-uniform translation rate on the protein biogenesis. Using the complete genomes of Escherichia coli and Bacillus subtilis we show that stretches of codons pairing to minor tRNAs form putative sites to locally attenuate translation; thereby the tendency is to cluster in near proximity whereas long contiguous stretches of slow-translating triplets are avoided. The presence of slow-translating segments positively correlates with the protein length irrespective of the protein abundance. The slow-translating clusters are predominantly located down-stream of the domain boundaries presumably to fine-tune translational accuracy with the folding fidelity of multidomain proteins. Translation attenuation patterns at highly structurally and functionally conserved domains are preserved across the species suggesting a concerted selective pressure on the codon selection and species-specific tRNA abundance in these regions.

Institut: Institut für Biochemie und Biologie
DDC-Sachgruppe: Biowissenschaften, Biologie
Dokumentart: c Postprint
Schriftenreihe: Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe, ISSN 1866-8372
Band Nummer: paper 132
Quelle: PLoS one 4 (2009), 4, Art. e5036, DOI: 10.1371/ journal.pone.0005036
Sprache: Englisch
Erstellungsjahr: 2009
Publikationsdatum: 16.07.2010
Bemerkung:
The article was originally published by PUBLIC LIBRARY SCIENCE:
PLoS one. - 4 (2009), 4, Art. e5036 (9 S.)
ISSN 1932-6203
DOI 10.1371/journal.pone.0005036
Lizenz: Dieses Werk ist unter einer Creative Commons-Lizenz lizenziert.
Lizenz-Logo  Creative Commons - Attribution 3.0 unported


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