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URN: urn:nbn:de:kobv:517-opus-9504
URL: http://opus.kobv.de/ubp/volltexte/2006/950/

Beck, Michael ; Hildebrandt, Niko ; Löhmannsröben, Hans-Gerd

Quantum dots as acceptors in FRET-assays containing serum

Dokument 1.pdf (299 KB)

Kurzfassung in Englisch

Quantum dots (QDs) are common as luminescing markers for imaging in biological applications because their optical properties seem to be inert against their surrounding solvent. This, together with broad and strong absorption bands and intense, sharp tuneable luminescence bands, makes them interesting candidates for methods utilizing Förster Resonance Energy Transfer (FRET), e. g. for sensitive homogeneous fluoroimmunoassays (FIA). In this work we demonstrate energy transfer from Eu3+-trisbipyridin (Eu-TBP) donors to CdSe-ZnS-QD acceptors in solutions with and without serum. The QDs are commercially available CdSe-ZnS core-shell particles emitting at 655 nm (QD655). The FRET system was achieved by the binding of the streptavidin conjugated donors with the biotin conjugated acceptors. After excitation of Eu-TBP and as result of the energy transfer, the luminescence of the QD655 acceptors also showed lengthened decay times like the donors. The energy transfer efficiency, as calculated from the decay times of the bound and the unbound components, amounted to 37%. The Förster-radius, estimated from the absorption and emission bands, was ca. 77 Å. The effective binding ratio, which not only depends on the ratio of binding pairs but also on unspecific binding, was obtained from the donor emission dependent on the concentration. As serum promotes unspecific binding, the overall FRET efficiency of the assay was reduced. We conclude that QDs are good substitutes for acceptors in FRET if combined with slow decay donors like Europium. The investigation of the influence of the serum provides guidance towards improving binding properties of QD assays.

SWD-Schlagwörter: Quantenpunkt , Lumineszenz , Serum , Europium , Immunoassay , Energietransfer , Fluoreszenz-Resonanz-Energie-Transfer
Freie Schlagwörter (Deutsch): Förster-Resonanz-Energie-Transfer
Freie Schlagwörter (Englisch): Quantum Dot , Luminescence , Serum, Europium , Immunoassay , Energy Transfer , FRET
RVK - Regensburger Verbundklassifikation: VE 9908
Collection: Universität Potsdam / Aufsätze (Pre- und Postprints) / Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie / Physikalische Chemie
Institut: Institut für Chemie
DDC-Sachgruppe: Chemie
Dokumentart: c Postprint
Schriftenreihe: Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe, ISSN 1866-8372
Bandnummer: paper 019
Quelle: Proceedings of SPIE, Vol. 6191 (2006), 61910X , pp. 234-241
Sprache: Englisch
Erstellungsjahr: 2006
Publikationsdatum: 29.09.2006
© 2006 Society of photo-Optical Instrumentation Engineers

This paper was published in:
Biophotonics and New Therapy Frontiers / Romualda Grzymala, Olivier Haeberle (eds.). - S. 234 - 241. - (Proceedings of SPIE ; 6191)
ISBN: 9780819462473
doi: 10.1117/12.662722

It is made available as an electronic reprint with permission of SPIE. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

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