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URN: urn:nbn:de:kobv:517-opus-45253
URL: http://opus.kobv.de/ubp/volltexte/2010/4525/

Szymanski, Jedrzej ; Jozefczuk, Szymon ; Nikoloski, Zoran ; Selbig, Joachim ; Nikiforova, Victoria ; Catchpole, Gareth ; Willmitzer, Lothar

Stability of metabolic correlations under changing environmental conditions in Escherichia coli : a systems approach

Dokument 1.pdf (16.640 KB) (SHA-1:dc53c10f1858e449b09b56ce0cdb22d8ea56552f)

Kurzfassung in Englisch

Biological systems adapt to changing environments by reorganizing their cellula r and physiological program with metabolites representing one important response level. Different stresses lead to both conserved and specific responses on the metabolite level which should be reflected in the underl ying metabolic network.

Methodology/Principal Findings:
Starting from experimental data obtained by a GC-MS based high-throughput metabolic profiling technology we here develop an approach that: (1) extracts network representations from metabolic conditiondependent data by using pairwise correlations, (2) determines the sets of stable and condition-dependent correlations based on a combination of statistical significance and homogeneity tests, and (3) can identify metabolites related to the stress response, which goes beyond simple ob servation s about the changes of metabolic concentrations. The approach was tested with Escherichia colias a model organism observed under four different environmental stress conditions (cold stress, heat stress, oxidative stress, lactose diau xie) and control unperturbed conditions. By constructing the stable network component, which displays a scale free topology and small-world characteristics, we demonstrated that: (1) metabolite hubs in this reconstructed correlation networks are significantly enriched for those contained in biochemical networks such as EcoCyc, (2) particular components of the stable network are enriched for functionally related biochemical path ways, and (3) ind ependently of the response scale, based on their importance in the reorganization of the cor relation network a set of metabolites can be identified which represent hypothetical candidates for adjusting to a stress-specific response.

Network-based tools allowed the identification of stress-dependent and general metabolic correlation networks. This correlation-network-ba sed approach does not rely on major changes in concentration to identify metabolites important for st ress adaptation, but rather on the changes in network properties with respect to metabolites. This should represent a useful complementary technique in addition to more classical approaches.

Freie Schlagwörter (Englisch): Small-world networks , saccharomyces-cerevisiae , trehalose synthesis , gene-expression , stress-response
Institut: Institut für Biochemie und Biologie
DDC-Sachgruppe: Biowissenschaften, Biologie
Dokumentart: c Postprint
Schriftenreihe: Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe, ISSN 1866-8372
Bandnummer: paper 147
Quelle: PLoS one 4 (2009), 10, Art. e7441, DOI: 10.1371/journal.pone.0007441
Sprache: Englisch
Erstellungsjahr: 2009
Publikationsdatum: 21.07.2010
The article was originally published by PUBLIC LIBRARY SCIENCE:
PLoS one. - 4 (2009), 10, Art. e7441 (15 S.)
ISSN 1932-6203
DOI 10.1371/journal.pone.0007441
Lizenz: Dieses Werk ist unter einer Creative Commons-Lizenz lizenziert.
Lizenz-Logo  Creative Commons - Attribution 3.0 unported

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