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URN: urn:nbn:de:kobv:517-opus-44335
URL: http://opus.kobv.de/ubp/texte_eingeschraenkt_verlag/2010/4433/


Blenau, Wolfgang ; Rotte, Cathleen ; Krach, Christian ; Balfanz, Sabine ; Baumann, Arnd ; Walz, Bernd

Molecular characterization and localization of the first tyramine receptor of the American cockroach (Periplaneta americana)

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Kurzfassung in Englisch

The phenolamines octopamine and tyramine control, regulate, and modulate many physiological and behavioral processes in invertebrates. Vertebrates possess only small amounts of both substances, and thus, octopamine and tyramine, together with other biogenic amines, are referred to as “trace amines.” Biogenic amines evoke cellular responses by activating G-protein-coupled receptors. We have isolated a complementary DNA (cDNA) that encodes a biogenic amine receptor from the American cockroach Periplaneta americana, viz., Peatyr1, which shares high sequence similarity to members of the invertebrate tyramine-receptor family. The PeaTYR1 receptor was stably expressed in human embryonic kidney (HEK) 293 cells, and its ligand response has been examined. Receptor activation with tyramine reduces adenylyl cyclase activity in a dose-dependent manner (EC50 350 nM). The inhibitory effect of tyramine is abolished by co-incubation with either yohimbine or chlorpromazine. Receptor expression has been investigated by reverse transcription polymerase chain reaction and immunocytochemistry. The mRNA is present in various tissues including brain, salivary glands, midgut, Malpighian tubules, and leg muscles. The effect of tyramine on salivary gland acinar cells has been investigated by intracellular recordings, which have revealed excitatory presynaptic actions of tyramine. This study marks the first comprehensive molecular, pharmacological, and functional characterization of a tyramine receptor in the cockroach.

Freie Schlagwörter (Englisch): Biogenic amine , cellular signaling , G-protein-coupled receptor , octopamine , salivary gland
Institut: Institut für Biochemie und Biologie
DDC-Sachgruppe: Biowissenschaften, Biologie
Dokumentart: c Postprint
Quelle: Neuroscience 162 (2009), 4, S. 1120 - 1133, DOI 10.1016/j.neuroscience.2009.05.066
Sprache: Englisch
Erstellungsjahr: 2009
Publikationsdatum: 27.08.2010
Bemerkung:
The article was originally published by:
Elsevier
Neuroscience. - 162 (2009), 4, S. 1120-1133
ISSN 0306-4522
DOI 10.1016/j.neuroscience.2009.05.066
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