Monitoring hormone-induced oxygen consumption in the salivary glands of the blowfly, Calliphora vicina, by use of luminescent microbeads

  • The salivary glands of the blowfly were injected with luminescent oxygen-sensitive microbeads. The changes in oxygen content within individual gland tubules during hormone-induced secretory activity were quantified. The measurements are based on an upgraded phase-modulation technique, where the phase shift of the sensor phosphorescence is determined independently from concentration and background signals. We show that the combination of a lock-in amplifier with a fluorescence microscope results in a convenient setup to measure oxygen concentrations within living animal tissues at the cellular level.

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Author details:Elmar Schmälzlin, Bernd Walz, Ingo Klimant, Bettina Schewe, Hans-Gerd LöhmannsröbenORCiDGND
URN:urn:nbn:de:kobv:517-opus-12206
Publication series (Volume number):Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe (paper 006)
Publication type:Postprint
Language:English
Publication year:2006
Publishing institution:Universität Potsdam
Release date:2007/01/26
Source:Sensors and Actuators B: Chemical. - ISSN 0925-4005. - 119 (2006), 1 , p. 251 - 254
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie
DDC classification:5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften
External remark:
first published in:
Sensors and Actuators B: Chemical. - ISSN 0925-4005. - 119 (2006), 1, p. 251 - 254
doi: 10.1016/j.snb.2005.12.024
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