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URN: urn:nbn:de:kobv:517-opus-10150
URL: http://opus.kobv.de/ubp/volltexte/2006/1015/


Niederkrüger, Matthias ; Salb, Christian ; Beck, Michael ; Hildebrandt, Niko ; Löhmannsröben, Hans-Gerd ; Marowsky, Gerd

Improvement of a fluorescence immunoassay with a compact diode-pumped solid state laser at 315 nm

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Dokument 1.pdf (734 KB)


Kurzfassung in Englisch

We demonstrate the improvement of fluorescence immunoassay (FIA) diagnostics in deploying a newly developed compact diode-pumped solid state (DPSS) laser with emission at 315 nm. The laser is based on the quasi-three-level transition in Nd:YAG at 946 nm. The pulsed operation is either realized by an active Q-switch using an electro-optical device or by introduction of a Cr4+:YAG saturable absorber as passive Q-switch element. By extra-cavity second harmonic generation in different nonlinear crystal media we obtained blue light at 473 nm. Subsequent mixing of the fundamental and the second harmonic in a β-barium-borate crystal provided pulsed emission at 315 nm with up to 20 μJ maximum pulse energy and 17 ns pulse duration. Substitution of a nitrogen laser in a FIA diagnostics system by the DPSS laser succeeded in considerable improvement of the detection limit. Despite significantly lower pulse energies (7 μJ DPSS laser versus 150 μJ nitrogen laser), in preliminary investigations the limit of detection was reduced by a factor of three for a typical FIA.

SWD-Schlagwörter: Immunoassay , Fluoreszenz-Resonanz-Energie-Transfer , Neodym-YAG-Laser
Freie Schlagwörter (Deutsch): 946 nm , 473 nm , 315 nm , gepulster DPSS Laser , sättigbarer Absorber
Freie Schlagwörter (Englisch): fluorescence immunoassay , 946 nm , 473 nm , 315 nm , pulsed DPSS laser
RVK - Regensburger Verbundklassifikation: VE 9908
Collection: Universität Potsdam / Aufsätze (Pre- und Postprints) / Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie / Physikalische Chemie
Institut 1: Institut für Chemie
Institut 2: Extern
DDC-Sachgruppe: Chemie
Dokumentart: c Postprint
Schriftenreihe: Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe, ISSN 1866-8372
Bandnummer: paper 016
Quelle: Proc. SPIE, Vol. 6380 (2006) 63800M
Sprache: Englisch
Erstellungsjahr: 2006
Publikationsdatum: 01.11.2006
Bemerkung:
© 2006 Society of Photo-Optical Instrumentation Engineers

This paper was published in:
Smart Medical and Biomedical Sensor Technology IV / Brian M. Cullum, J. Chance Carter (eds.), Proceedings of SPIE, Vol. 6380 (2006)
ISBN: 0-8194-6478-3

It is made available as an electronic reprint with permission of SPIE. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purpose, or modification of the content of the paper are prohibited.


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